In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:
No
Cytogenetic Analysis:
The chromosome number distribution is bimodal with 50% of the cells near diploid （mode = 44 chromosomes） and 50% tetraploid （mode = 88）. Both populations consistently showed seven marker chromosomes: 5p+, del（6）（p11）, 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Subculturing:
Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subc*tion Ratio: 1:2 to 1:5 Medium Renewal: Every 2 to 3 days
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004 recommended serum:ATCC 30-2020 derivative:ATCC CRL-9519
References:
3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990
會(huì)員1.png)
