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為了獲得更理想的ELISA實驗結果，請您

1、在ELISA實驗過程中，請配制標準品及工作液A和B，以避免由于不準確稀釋而造成的濃度誤差；

2、在ELISA實驗過程中，酶標板在溫育時應加膜覆蓋以避免液體蒸發；

3、在ELISA實驗過程中，檢測液A和檢測液B，以及底物溶液在使用前，應置于37℃溫育30分鐘待用；

4、在ELISA實驗過程中，洗板后應盡快進行下一步操作，避免酶標板長時間處于干燥狀態。

In order to obtain the optimal ELISA results

1．During ELISA assay operation, Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction and avoid foaming and mix gently until the crystals have compley dissolved;

2．During ELISA assay operation, Proper adhesion of plate sealers during incubation steps is necessary;

3．During ELISA assay operation, Substrate, Detection Reagent A and Detection Reagent B should be incubate for 30 min at 37℃ before use;

4．During ELISA assay operation, After the every step of aspiration / wash, please do not delay entering into the next step in order not to let the assay plate "too dry".