當(dāng)前位置：賽默飛世爾科技（中國(guó)）有限公司>>蛋白分析試劑>>蛋白檢測(cè)>>Tango™ 開(kāi)放啟用模型

Tango™ 開(kāi)放啟用模型

返回列表頁(yè)

參考價(jià)

面議

具體成交價(jià)以合同協(xié)議為準(zhǔn)

產(chǎn)品型號(hào)Thermo Scientific

品牌

廠商性質(zhì)其他

所在地

在線詢價(jià) 收藏產(chǎn)品查看聯(lián)系電話

聯(lián)系方式：查看聯(lián)系方式

更新時(shí)間：2023-11-06 19:27:31瀏覽次數(shù)：164次

聯(lián)系我時(shí)，請(qǐng)告知來(lái)自環(huán)保在線

產(chǎn)品分類(lèi) 品牌分類(lèi)

  蛋白分析試劑

蛋白質(zhì)譜檢材制備

親和素-結(jié)合產(chǎn)品

蛋白檢測(cè)

透析及脫鹽

蛋白純化

蛋白檢測(cè)系統(tǒng)

蛋白樹(shù)脂

蛋白提取

生物活性小分子

氨基酸分析試劑和附件

抗體親和純化緩沖液

感受態(tài)細(xì)胞

基因報(bào)告系統(tǒng)

免疫組織化學(xué)試劑盒和試劑

親和素產(chǎn)品

SILAC 同位素標(biāo)記試劑和試劑盒

全部產(chǎn)品列表

暫無(wú)信息

賽默飛世爾科技（中國(guó)）有限公司

經(jīng)營(yíng)模式：其他

商鋪產(chǎn)品：2514條

所在地區(qū)：

詢價(jià) 給他留言

產(chǎn)品簡(jiǎn)介

Tango™ 開(kāi)放啟用模型

詳細(xì)介紹

The Tango™ Assay technology combines the benefits of the Tango™ assay platform with the highly accurate, sensitive, and live cell GeneBLAzer® Beta-lactamase reporter system. The Tango™ assay measures protein-protein interactions in live mammalian cells. One of the proteins of interest is anchored to cell surface either by its own transmembrane domains or through fusion to CD8 using a "bait" vector provided in this kit. The "bait" vector also provides at its intracellular C-terminus, an in-frame fusion to an exogenous transcription factor, GAL4-VP16. Interposed between the protein of interest and the transcription factor is a specific cleavage sequence for a non-native protease. This chimeric receptor protein is expressed in a cell line containing the beta-lactamase reporter gene responsive to the transcription factor. The "prey" vectors allow an interacting protein to be expressed as a protease fusion protein that recognizes and cleaves the site between the anchored membrane protein and transcription factor. The assay is performed by adding a stimulus to the growing cells for a defined period and measuring the activity of the reporter gene. Activation of the reporter gene provides a quantifiable measurement of the degree of interaction between the anchored membrane protein and the protease-tagged interaction partner and is unaffected by other signaling pathways in the cell, providing an exquisitely specific readout of protein-protein interaction.

For Research Use Only. Not for use in diagnostic procedures.