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Serotonin 5-HT1A AequoScreen; cell line

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產品簡介

Stable, recombinant AequoScreen® cell line expressing aequorin and the Serotonin receptor 5-HT1A, human recombinant in CHO-K1 host cells.

詳細介紹

PerkinElmer’s AequoScreen^® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 10⁶ cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.

The AequoScreen technology is a generic GPCR technology that can be used with G_s, G_i, and G_q-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Features:

Fully automatable and miniaturizable to 1536
Enables reduction in reagents and consumable costs
High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
Robust assay (Z′, CV values, solvent resistance)
Reduces the number of false positives from autofluorescent compounds in screening libraries
Option to work with cells in suspension to increase throughput
Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

PerkinElmer’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.

規格

檢測目標類	GPCR
檢測目標類型	Cell line
檢測驗證	Binding, Calcium Luminescence
G-Alpha天然受體	Gi/Go
宿主細胞	CHO-K1
產品品牌名稱	AequoScreen
受體類型	5-Hydroxytryptamine (Serotonin)
第二信使釋放	Calcium flux
運輸條件	干冰
特殊訂購信息	Requires accepted Terms and Conditions. Additional agreement needed for commercial use.
治療領域	Central Nervous System, Cardiovascular, Inflammation, Metabolic, Gastrointestinal
產品尺寸	5 million cells