LysoBrite 溶酶體近紅外熒光探針，用于標記活細胞的溶酶體。專有的溶解性染料可能通過溶酶體pH梯度選擇性地積聚在溶酶體中。溶質指示劑是疏水性化合物，易于滲透完整的活細胞，并在進入細胞后被捕獲在溶酶體中。進入溶酶體后，LysoBrite 試劑的熒光明顯增強。可以使用熒光成像，高含量成像，酶標儀熒光測定法或流式細胞術檢測熒光。 LysoBrite 橙色，紅色，深紅色和NIR試劑具有很高的光穩定性和優異的細胞保留性，可用于各種研究，包括細胞粘附，趨化性，多藥耐藥性，細胞活力，細胞凋亡和細胞毒性。它們適用于增殖和非增殖細胞，可用于懸浮細胞和貼壁細胞。溶酶體是細胞器，其含有酸水解酶以分解廢物和細胞碎片。溶酶體消化多余的或破舊的細胞器，食物顆粒和吞噬的病毒或細菌。溶酶體周圍的膜允許消化酶在pH4.5下工作。與微堿性胞質溶膠（pH 7.2）相比，溶酶體的內部是酸性的（pH 4.5-4.8）。溶酶體通過質子泵和氯離子通道從細胞質中泵送質子穿過膜來維持這種pH差異。

2.百螢 LysoBrite溶酶體近紅外熒光探針參數

Ex(nm) 636

Em(nm) 651

分子量 730.89

溶劑 DMSO

存儲條件在-15℃以下保存，避光防潮

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