Pierce™ Streptavidin Agarose Columns, 1 mL

返回列表頁

參考價(jià)

面議

具體成交價(jià)以合同協(xié)議為準(zhǔn)

產(chǎn)品型號(hào)Thermo Scientific

品牌

廠商性質(zhì)0

所在地

在線詢價(jià) 收藏產(chǎn)品查看聯(lián)系電話

聯(lián)系方式：客服查看聯(lián)系方式

更新時(shí)間：2023-08-08 08:11:05瀏覽次數(shù)：133次

聯(lián)系我時(shí)，請(qǐng)告知來自環(huán)保在線

產(chǎn)品分類 品牌分類

  蛋白分析試劑

蛋白質(zhì)譜檢材制備

親和素-結(jié)合產(chǎn)品

蛋白檢測(cè)

透析及脫鹽

蛋白純化

蛋白檢測(cè)系統(tǒng)

蛋白樹脂

蛋白提取

生物活性小分子

氨基酸分析試劑和附件

抗體親和純化緩沖液

感受態(tài)細(xì)胞

基因報(bào)告系統(tǒng)

免疫組織化學(xué)試劑盒和試劑

親和素產(chǎn)品

SILAC 同位素標(biāo)記試劑和試劑盒

全部產(chǎn)品列表

暫無信息

賽默飛世爾科技（中國(guó)）有限公司集團(tuán)

經(jīng)營(yíng)模式：

商鋪產(chǎn)品：2508條

所在地區(qū)：

聯(lián)系人：客服（客服）

詢價(jià) 給他留言

產(chǎn)品簡(jiǎn)介

Pierce™ Streptavidin Agarose Columns, 1 mL

詳細(xì)介紹

Thermo Scientific Pierce Streptavidin Agarose Columns are 5-mL capacity columns containing 1 mL streptavidin agarose, for use in a variety of biotin-binding affinity purification methods

Features of Streptavidin Agarose:

• Streptavidin—purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
• Agarose resin—support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• Standard capacity—this variety of beads has a normal load of immobilized streptavidin, providing a binding capacity of 1 to 3 mg of biotinylated BSA per mL of resin

Properties of crosslinked 6% beaded agarose (CL-6B):
• Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
• Average Particle Size: 45 to 165 microns
• Exclusion Limit: 10,000 to 4,000,000 daltons
• Maximum Volumetric Flow Rate: approx. 1 mL/minute (for 1 cm diameter column)
• Maximum Linear Velocity: 30 cm per hour
• Maximum Pressure: less than 25psi (1.5 bar)

Applications:
• Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies
• Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent
• Isolation of cell-surface glycoproteins using biotinylated Concanavalin A
• Recovery of single-stranded biotinylated DNA for dideoxy sequencing
• Streptavidin-coated plates used for direct solid-phase sequencing
• In vitro mutagenesis with streptavidin-coated plates
• DNA fingerprinting with streptavidin-coated plates

Pierce Streptavidin Agarose consists of purified recombinant streptavidin that has been covalently immobilized onto high-quality crosslinked 6% beaded agarose. Streptavidin is a tetrameric biotin-binding protein. The agarose beads have physical and chemical properties that enable them to be used in a variety of batch- or column-type affinity procedures. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

Related Products
Pierce™ Streptavidin Agarose
Pierce™ High Capacity Streptavidin Agarose
Pierce™ Streptavidin Plus UltraLink™ Resin

For Research Use Only. Not for use in diagnostic procedures.