Mouse anti-CD34
Catalog Number : HZ342113 Quantity size : 0.1ml （dilute with pH 7.4
0.01 M PBS or antibody diluent ）
Background: CD34 is a heavily glycosylated, transmembrane glycoprotein that is
expressed on the surface of lymphohematopoietic stem and progenitor cells,
small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult
nervous tissue. CD34 antigen expression is highest in the most primitive stem cells
and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen
is also present on capillary endothelial cells and on bone marrow stromal cells. The
CD34 cytoplasmic domain has an intracellular domain that contains consensus sites
for activated protein kinase C （PKC） phosphorylation as well as serine, threonine and
tyrosine phosphorylation consensus sites.
Specificity :
– CD34 is a mouse monoclonal antibody raised agianst blast cells of a chronic
myeloid leukemia patient..
– specific for CD34 antibody of human,Rat
– use for western blotting, elisa, immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 105-120kDa.
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least one month and for
greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M
PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃.
Important Note: This product as supplied is intended for research use only, not for
use in human, therapeutic or diagnostic applications.