人褪黑素(MT)ELISA試劑盒
實驗原理：
本試劑盒用于測定人血清，組織及相關(guān)液體樣本中褪黑素 (MT)的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中人褪黑素 (MT)水平。用純化的人褪黑素 (MT) 抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入褪黑素 (MT)，再與HRP標(biāo)記的褪黑素 (MT)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的褪黑素 (MT)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中人褪黑素 (MT)濃度。
Human melatonin(MT)ELISA Kit
Principle of the assay
The kit allows for the determination of MT concentrations in Human serum, blood plasma, and other biological fluids.Assay Human MT level in the sample，use Purified Human MT to coat microtiter plate wells, make solid-phase antibody, then add MT to wells, Combined MT antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司
會員1.png)