大鼠促甲狀腺激素（TSH）酶聯(lián)免疫分析(ELISA)試劑盒
實(shí)驗(yàn)目的：本試劑盒僅供研究使用，用于測(cè)定大鼠血清，血漿及相關(guān)液體樣本中促甲狀腺激素（TSH）的含量。
實(shí)驗(yàn)原理：本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中大鼠促甲狀腺激素（TSH）水平。用純化的大鼠促甲狀腺激素（TSH）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入促甲狀腺激素（TSH），再與HRP標(biāo)記的促甲狀腺激素（TSH）抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的大鼠促甲狀腺激素（TSH）呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中大鼠促甲狀腺激素（TSH）濃度。
Rat thyroid-stimulating hormone (TSH) ELISA Kit.
Purpose
This kit allows for the determination of TSH concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat TSH level in the sample，use Purified Rat TSH to coat microtiter plate wells, make solid-phase antibody, then add TSH to wells, Combined TSH antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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