26S Proteasome Fraction抗體說明書

FROM: GeneTex

Catalog Number: GTX80879

Background: Mammalian 26S proteasome purified through a specific series of specialized chromatographicsteps yields a partially purified fraction containing 26S proteasome and no conjugation enzymes（E1, E2s, and E3s）. This stable fraction is preferred for the degradation of pre-formed ubiquitinproteinconjugates by the 26S proteasome since highly purified 26S is costly and more labile.26S concentration is estimated based on analytical SEC at 1500 kDa and quantitation of the 20Score particle activity using a fluorogenic peptide substrate.

Item   : 26S Proteasome Fraction

Quantity size:0.2ml

Format: Estimated 26S concentration = X nM. Concentration will vary with Lot#.Buffer: 50 mM Hepes pH 7.6, 1 mM MgATP, 1 mM DTT,150 mM NaCl, 10 % glycerol.

Species:  rabbit

Host   :  rabbit reticulocytes

Source/Expression System:  rabbit

References

Driscoll J. and Goldberg A.L. （1990） J. Biol. Chem. 265:4789-4792 Ganoth D., et al. （1988） J. Biol. Chem. 263:12412-12419 Voges D., et al. （1999） Ann. Rev. Biochem. 68:1015-1068 Waxman L., et al. （1987） J. Biol. Chem. 262:2451-2457

Specifications/Features

For best results, aliquot and freeze after first set of experiments to avoidmultiple freeze/thaw cycles. Typical concentrations for use are 5-50 nMdepending on conditions.

26S Proteasome Fraction抗體說明書

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