Anti-ERCC1抗體說(shuō)明書(shū)
Quantity size : 100ul （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Xeroderma pigmentosum （XP） is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF , which is also designated ERCC4 or ERCC11, is a protein that associates directly with the excision repair crosscomplementing 1 （ERCC1） factor . ERCC1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair . The ERCC1-XPF endo-nuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.
Anti-ERCC1抗體說(shuō)明書(shū)
pecificity :
– Anti-ERCC1 is a rabbit monoclonal antibody unconjugated
– specific for ERCC1 of human, mouse and rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 38 kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-ERCC1抗體說(shuō)明書(shū)
