| Catalogue Number | CYT-388 |
| Synonyms | IFN-b2, B cell differentiation factor （BCDF）, BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor. |
| Introduction | Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis. |
| Description | Interleukin-6 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton.
The IL6 is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein was lyophilized without any additives. |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-6 in 100mM acetic acid to 1.0 mg/mL and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser. |
| Biological Activity | The ED50= 0.03-0.1 ng/ml corresponding to a specific activity of 10,000,000-34,000,000IU/mg. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. A concentration range of 0.1-10.0 ng/mL is effective for most in vitro applications. |
| Protein content | Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
