| Catalogue Number | CYT-211 |
| Synonyms | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. |
| Introductionn | IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinay by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Description | Interleukin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton.
The IL-4 is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated （1mg/ml） solution in PBS, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. |
| Purity | Greater than 98.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. |
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Cys-Asp. |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg. |
| Protein content | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.
2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. |
| References | 1. Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation1
Publication:The Journal of Immunology, 2006, 177: 6695-6704.Copyright ? 2006 by The American Association of Immunologists, Inc.
Link:http://www.jimmunol.org/content/177/10/6695.full
2.Title:CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signals in the immunological synapse
Publication:Published online before print June 27, 2005, doi: 10.1073/pnas.0501050102 PNAS July 5, 2005 vol. 102 no. 27 9583-9588
Link:http://www.pnas.org/content/102/27/9583.full
3.Title:A Network Modeling Approach to Analysis of the Th2 Memory Responses Underlying Human Atopic Disease
Publication:doi: 10.4049/?jimmunol.0804125 The Journal of Immunology May 15, 2009 vol. 182 no. 10 6011-6021
Link:http://www.jimmunol.org/content/182/10/6011.full
4.Title:Defective IL-10 production in severe phenotypes of Crohn’s disease
Publication:Published online before print February 23, 2009, doi: 10.1189/jlb.1108698 May 2009 Journal of Leukocyte Biology vol. 85 no. 5 896-903
Link:http://www.jleukbio.org/content/85/5/896.full
5.Title:Adenosine deaminase potentiates the generation of effector, memory, and regulatory CD4+ T cells .
Publication:Published online before print October 19, 2010, doi: 10.1189/jlb.1009696 January 2011 Journal of Leukocyte Biology vol. 89 no. 1 127-136 .
Link:http://www.jleukbio.org/content/89/1/127.full
6.Title:Glutamate Released by Dendritic Cells as a Novel Modulator of T Cell Activation/
Publication:
Link:http://journal.9med.net/qikan/article.php?id=367793
7.Title:Airway Epithelial Cells Regulate the Functional Phenotype of Locally Differentiating Dendritic Cells: Implications for the Pathogenesis of Infectious and Allergic Airway Disease.
Publication: The Journal of Immunology January 1, 2009 vol. 182 no. 1 72-83
Link:http://www.jimmunol.org/content/182/1/72.full |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
