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*17/IL 17 Human 產品編號：CYT-250

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*17/IL 17 Human產品編號：CYT-250
產品規格：5μg/25μg/1mg

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*17/IL 17 Human

產品編號：CYT-250
產品規格：5μg/25μg/1mg

Catalogue Number	CYT-250
Synonyms	CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.
Introduction	IL17 is a proinflammatory cytokine produced by activated T cells. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. Interleukin-17 can stimulate the expression of IL6 and cyclooxygenase-2 （PTGS2/COX-2）, as well as enhance the production of nitric oxide （NO）. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Description	Interleukin-17A Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids （2 chains of 132 aa） and having a molecular mass of 31kDa. The IL-17 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	Sterile filtered lyophilized （freeze-dried） powder, with 20mM Citrate, 0.1M NaCl, pH4.5.
Solubility	It is recommended to reconstitute the lyophilized Interleukin 17 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Interleukin 17A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL17 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by SDS-PAGE.
Biological Activity	The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximay 2ng/ml corresponding to a Specific Activity of 500,000IU/mg.
Protein content	Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.