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重組人肝細(xì)胞生長(zhǎng)因子/HGF Human 產(chǎn)品編號(hào)：CYT-244

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重組人肝細(xì)胞生長(zhǎng)因子/HGF Human產(chǎn)品編號(hào)：CYT-244
產(chǎn)品規(guī)格：2μg/10μg/1mg

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重組人肝細(xì)胞生長(zhǎng)因子/HGF Human

產(chǎn)品編號(hào)：CYT-244
產(chǎn)品規(guī)格：2μg/10μg/1mg
 

Catalogue Number CYT-244
Synonyms Scatter Factor （SF）, Hepatopoietin （HPTA）, HGF, HGFB, F-TCF.
Introduction Hepatocyte Growth Factor （HGF） is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.
Description Hepatocyte Growth Factor Human Recombinant produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain containing 692 a.a and having a total molecular mass of 78.0 KDa.
The HGF is purified by proprietary chromatographic techniques.
Source Insect cells.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation The sterile protein powder （1 mg/ml） is lyophilized from a solution containing 50mM acetic acid.
Solubility It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. For further dilutions should be made into buffer containing 250mM NaCl.
Stability Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCK
AFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGT
VSITKSGIKCQPWSSMIPHEHSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRY
EVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPD
KGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECI
QGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGS
ESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSM
WDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISR
CEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGS
likeSWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEG
SDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLR
VAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKM
RMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS.
Biological Activity The activity was assayed for scattering activity in the MDCK cell assay. The ED50 for this effect is typically at 2.0-5.0 ng/ml （200,000-500,000U/mg）.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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