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IL 7 Human/*-7

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IL 7 Human/*-7產品編號：CYT-254
產品規格：2μg/10μg/1mg

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IL 7 Human/*-7

產品編號：CYT-254
產品規格：2μg/10μg/1mg

Synonyms	Lymphopoietin 1 （LP-1）, pre-B cell factor, IL-7.
Introduction	IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor （HGF） form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V（D）J rearrangement of the T cell receptor beta （TCRB） during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.
Description	Interleukin-7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17412 Dalton. The IL-7 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	Lyophilized from a concentrated （1mg/ml） solution in water containing no additives.
Solubility	It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 98.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Amino acid sequence	MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH.
Biological Activity	The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is < 0.5ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.
Protein content	Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.