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IGF1 Rat/重組大鼠胰島素樣生長因子-I 產品編號：CYT-289

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IGF1 Rat/重組大鼠胰島素樣生長因子-I 產品編號：CYT-289
產品規格：10μg/50μg/1mg

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IGF1 Rat/重組大鼠胰島素樣生長因子-I

產品編號：CYT-289
產品規格：10μg/50μg/1mg

Synonyms	Somatomedin C, IGF-I, IGFIA, IGF1.
Introduction	The somatomedins, or insulin-like growth factors （IGFs）, comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone （GH; MIM 139250）. Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' （Daughaday et al., 1972）. Three main somatomedins have been characterized: somatomedin C （IGF1）, somatomedin A （IGF2; MIM 147470）, and somatomedin B （MIM 193190）（Rotwein, 1986; Rosenfeld, 2003）.
Description	Insulin-like Growth Factor I Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7687 Dalton. IGF-I is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	The protein was lyophilized with no additives.
Solubility	It is recommended to reconstitute the lyophilized IGF1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 98.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Biological Activity	The ED50, was calculated 2 methods1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be < 30 ng/ml. 2. Type 1 IGF receptor binding assay ED50 was found to be <10 ng/ml.