抗角蛋白絲聚集素絲集蛋白抗體(AFA)檢測試劑盒英文Human anti-filaggrin antibody,AFA ELISA Kit齊一生物科技(上海)有限公司以真誠、主動、周到、規范、熱情的服務很快得到廣大客戶的*好評.公司秉承“適應多樣化的需求,提供專業化的服務"的經營理念,為客戶提供*的服務
抗角蛋白絲聚集素絲集蛋白抗體(AFA)檢測試劑盒類別:酶聯免疫分析檢測試劑盒
規格:96T/48T
包裝:盒
檢測原理:采用雙抗夾心法
保質期:六個月
產品保存條件:2-8°C
應用范圍:僅供科研研究實驗使用
樣品形式:血清/血漿/組織勻漿/細胞培養上清//其它生物液體(具體情況請咨詢)
標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存 過程 中 如有沉淀形成,應再次離心。
2.血漿:應根據標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉/ 分)。仔細收集上清。 檢測細胞內的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內成份。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存備用。標本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求:
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
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Service response time (repair): 7-10 days turnaround time
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: not applicable
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: not included
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage"
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SC-His-Leu (Powder) SC-His-Leu 培養基 30.0 g
SC-Cys-Met (Powder) SC-Cys-Met 培養基 30.0 g
SC-Trp-Ura (Powder) SC-Trp-Ura 培養基 30.0 g
SC-Ura (Powder) SC-Ura 培養基 100 g
SC-Ura (Powder) SC-Ura 培養基 30 g
SC-Trp (Powder) SC-Trp 培養基 30.0 g
SC-Lys (Powder) SC-Lys 培養基 30.0 g
SC-Leu (Powder) SC-Leu 培養基 30.0 g
SC-His (Powder) SC-His 培養基 30.0 g
SC-Ade (Powder) SC-Ade 培養基 30.0 g
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MM-PG Media MM-PG 500 ml
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BMMY-PG Media BMMY-PG 500 ml
BMGY-PG Media BMGY-PG 500 ml
MMH-PG Media MMH-PG 500 ml
MD-PG Media MD-PG 500 ml
RDHB-PG Plates RDHB-PG 平板 25 each
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EMM (Case)(Pouches) EMM(袋裝) 100x1.0 L
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EMM (Powder) EMM(粉末) 1 kg(2.2lb)
EMM (Powder) EMM(粉末) 5 kg(11lb)
SP Supplements (Powder) SP Supplements (粉末) 10 g
YEA (Powder) YEA (粉末) 227 g(0.5lb)
YEA (Powder) YEA (粉末) 454 g(1lb)
YES (Powder) YES(粉末) 454 g(1lb)
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YE(Powder) YE(粉末) 1 kg(2.2lb)
YES Agar (Powder) YES 含瓊脂(粉末) 1 kg(2.2lb)
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