培養基和添加劑 | Eagle MEM (Invitrogen, 11090081) 87 ml FBS (Biochrom, S4115) 10 ml Glutamax（invitrogen 35050） 1 ml SODIUM PYRUVATE SOL（invitrogen 11360070） 1 ml Non-essential Amino Acids, 100? (Invitrogen, 11140) 1 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。 |
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