細胞名稱

RW.4小鼠胚胎干細胞RW.4（干細胞庫保藏）

描述

小鼠*性胚胎干細胞，每支細胞量1×10⁶

動物種別

小鼠，129X1/SvJ品系

性別

雄

組織來源

小鼠胚胎內細胞團

形態

球形克隆

培養基和添加劑

mES*培養液配方（600 ml）： DMEM (Invitrogen 12430) 497 ml ES級FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl（終濃度為1000U/ml） β-Mer (Invitrogen 21985) 1.5 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應限制

REFERENCE

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