細(xì)胞名稱

F9小鼠畸胎瘤細(xì)胞

描述

在*酸和聯(lián)丁酰基cAMP刺激下，F(xiàn)9細(xì)胞分化成體壁內(nèi)皮層。 分化的細(xì)胞合成血漿酶原活化因子，粘層蛋白和IV 型膠原質(zhì)。 只有在經(jīng)過*酸處理后細(xì)胞上的cAMP才是活化的。 細(xì)胞中有三個(gè)拷貝β1粘合素基因。 檢測(cè)表明肢骨發(fā)育畸形病毒(鼠痘)陰性。

動(dòng)物種別

小鼠

性別

***

組織來源

睪丸；畸胎癌；睪丸畸胎癌

形態(tài)

上皮細(xì)胞

培養(yǎng)基和添加劑

DMEM培養(yǎng)基(GIBCO,貨號(hào)12800017，添加NaHCO3 1.5g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Strickland S , et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719 Strickland S , Mahdavi V . The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238 Stephens LE , et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677 Berstine EG , et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649