小鼠黃嘌呤ELISA試劑盒FOR RESEARCH USE ONLY
Drug Names
Generic Name：小鼠黃嘌呤ELISA試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：360ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

其它進口產(chǎn)品11QD0500    DNA-OFF    DNA去除試劑    500ml
    N-Lauroylsarcosine    N-十二烷基肌氨酸鈉    50g
    N-Lauroylsarcosine    N-十二烷基肌氨酸鈉    100g
    N-Lauroylsarcosine    N-十二烷基肌氨酸鈉    250g
    Isopropyl Alcohol    異戊醇    25ml
    Isopropyl Alcohol    異戊醇    100ml
    Isopropyl Alcohol    異戊醇    250ml
112450204    Rnase/Dnase-Free Water    Rnase/Dnase-Free 水    500ml
112420104    Depc-Treated Water    DEPC處理的水    200ml
112430304    Endotoxin-Free Water    無內(nèi)毒素水    100ml
蛋白質(zhì)純化            
116550400    FastPROTEIN Blue Matrix    FastPROTEIN細(xì)菌蛋白質(zhì)提取基質(zhì)    50×2ml
116550500    FastPROTEIN Blue Matrix    FastPROTEIN細(xì)菌蛋白質(zhì)提取基質(zhì)    100×2ml
116550600    FastPROTEIN Red Matrix    FastPROTEIN酵母蛋白質(zhì)提取基質(zhì)    50×2ml
116550700    FastPROTEIN Red Matrix    FastPROTEIN酵母蛋白質(zhì)提取基質(zhì)    100×2ml
克隆和轉(zhuǎn)化            
克隆篩選            
    Ampicillin    氨芐青霉素    5g
    Ampicillin    氨芐青霉素    25g
    Ampicillin    氨芐青霉素    100g
11IPTG0001    IPTG    IPTG    1g
11IPTG0005    IPTG    IPTG    5×1g
11IPTG0010    IPTG    IPTG    10×1g
11XGAL0100    X-Gal    X-Gal    100mg
11XGAL0500    X-Gal    X-Gal    5×100mg
11XGAL1000    X-Gal    X-Gal    1g
11XGAL5000    X-Gal    X-Gal    5×1g
E.coli 轉(zhuǎn)化            
112150200    Transform&Grow Bacterial Transformation Kit     細(xì)菌轉(zhuǎn)化和生長試劑盒    50preps
113030021    SOB Medium(Capsules)    SOB培養(yǎng)基（膠囊）    454g
113031012    SOC Medium    SOC培養(yǎng)基    227g
113301100    Agrobacterium Transformation Kit    土壤農(nóng)桿菌轉(zhuǎn)化試劑盒    25preps
S.cerevisiae 轉(zhuǎn)化            
112100200    EZ-Yeast Transformation Kit     EZ-Yeast 酵母轉(zhuǎn)化試劑盒    200preps
112200200    Alkali-Cation Yeast Transformation Kit    堿離子法酵母轉(zhuǎn)化試劑盒    250preps
端粒酶檢測，DNA甲基化，雜交            
11S7700KIT    TRAPeze® omerase    TRAPeze® 端粒酶檢測試劑盒    112 assays
11S7705    TRAPeze® 1XChaps Lysis    TRAPeze® 1XChAPS 裂解緩沖液    10 mL
11S7820KIT    CpGenone™ DNA Modif Kit    CpGenone™ DNA 重亞硫酸鹽修飾試劑盒    100 rxns
11S7821    Univer. Meth. DNA (10ug)    人甲基化基因組DNA 0.1μg/L    10 µg
11S1120DIG    FRAGILE X CHEMI™ Probe    FRAGILE X CHEMI™ 高辛標(biāo)記DNA探針和雜交試劑盒合用檢測FMR-1基因中的(CGG)重復(fù)片段    10 tests
11S4250KIT    Sure Blot® CHEMI™ Hybrid Detection Kit    Sure Blot® CHEMI™ 雜交檢測試劑盒    1 kit
11SSC20X01    SSC Easy Sol    檸檬酸鈉鹽緩沖液，雜交實驗DNA轉(zhuǎn)膜過程DNA從膠到膜的緩沖液    5 L
11SSC20X02    SSC Easy Sol    檸檬酸鈉鹽緩沖液，雜交實驗DNA轉(zhuǎn)膜過程DNA從膠到膜的緩沖液    2 x 5 L
11FIXO0125    Fixogum Rubber Cement    雜交過程中玻璃蓋片周圍的氣體密封圈    125 mL
11RIST1376    Antifade    Antifade    1ml
11RIST1373    Protein Digesting Enzyme    蛋白質(zhì)消化酶，用來消化石蠟包埋切片，福爾馬林浸泡組織中交聯(lián)酶以利于FISH探針進入細(xì)胞核    100mg
轉(zhuǎn)染            
siRNA,寡核苷酸，肽段轉(zhuǎn)染            
11PENA0500    Activated Penetratin 1 Peptide    Activated Penetratin 1 肽段，寡核苷酸，SiRNA轉(zhuǎn)染試劑    500ug
11PENB0500    Biotinylated activated penetratin 1 peptide    化 activated penetratin 1肽段，寡核苷酸，SiRNA 轉(zhuǎn)染試劑    500ug
113303020    NanoPortamin™ DNA Transfection Reagent    NanoPortamin™ DNA轉(zhuǎn)染試劑    200 µL
113303100    NanoPortamin™ DNA Transfection Reagent    NanoPortamin™ DNA轉(zhuǎn)染試劑    1 mL
113304050    NanoPorter™ DNA Transfection Kit    NanoPorter™ DNA轉(zhuǎn)染試劑    500 µL
113304100    NanoPorter™ DNA Transfection Kit    NanoPorter™ DNA轉(zhuǎn)染試劑    1 mL
113305020    SmallPorter Nanofectin SiRNA Transfection Kit    SmallPorter Nanofectin SiRNA轉(zhuǎn)染試劑    200 µL
113305100    SmallPorter Nanofectin SiRNA Transfection Kit    SmallPorter Nanofectin SiRNA轉(zhuǎn)染試劑    1 mL
Protein transfection蛋白質(zhì)轉(zhuǎn)染            
11GDSC0025    Chariot    Chariot蛋白質(zhì)多肽轉(zhuǎn)染試劑    25reactions
11GDSC0100    Chariot    Chariot蛋白質(zhì)多肽轉(zhuǎn)染試劑    100reactions
篩選試劑            
    G418    G418    100mg
    G418    G418    250mg
    G418    G418    1g
    G418    G418    5g
 電泳             
 11AGAR0025     Agarose, High Resolution     瓊脂糖，高溶解度     25 g 
 11AGAR0050     Agarose, High Resolution     瓊脂糖，高溶解度     50 g 
 11AGAL0050      Agarose, Low Melting Point     瓊脂糖，低溶點     50 g 
 11AGAH0100     Agarose, Mol. Bio. Grade, low EEO     瓊脂糖，分子生物學(xué)級別    100 g 
11AGAH0500     Agarose, Mol. Bio. Grade, low EEO     瓊脂糖，分子生物學(xué)級別    500 g
04800655    Acrylamide/Bis Premix 19:1    丙烯酰胺/甲叉雙丙烯酰胺 19:1    30 g
04800656    Acrylamide/Bis Premix  19:1    丙烯酰胺/甲叉雙丙烯酰胺 19:1    200 g
04800657    Acrylamide/Bis Premix  29:1    丙烯酰胺/甲叉雙丙烯酰胺 29:1    30 g
04800658    Acrylamide/Bis Premix  29:1    丙烯酰胺/甲叉雙丙烯酰胺 29:1    200 g
04800659    Acrylamide/Bis Premix  37.5:1    丙烯酰胺/甲叉雙丙烯酰胺 37.5:1    30 g
04800660    Acrylamide/Bis Premix 37.5:1    丙烯酰胺/甲叉雙丙烯酰胺 37.5:1    200 g
    Acrylamide    丙烯酰胺    100 g
    Acrylamide    丙烯酰胺    500 g