人皮膚成纖維細(xì)胞CCC-HSF-1本公司代理美國ATCC，理德DSMZ，ScienCell德國細(xì)胞庫，ECACC,ICLC,DSMZ的細(xì)胞株以及菌株等品牌細(xì)胞。齊一生物大量庫存ATCC 細(xì)胞，癌細(xì)胞，正常細(xì)胞，*，質(zhì)量保證，公司秉承“誠信、專業(yè)、高效"的經(jīng)營理念為廣大客戶服務(wù)。歡迎各位老師！齊一生物科技(上海）有限公司：。

人皮膚成纖維細(xì)胞CCC-HSF-1一、細(xì)胞操作參考書
二、生長特性：貼壁    懸浮
三、細(xì)胞生長條件：
培養(yǎng)基                  90%              （GIBCO）
血清              10%  FBS（GIBCO）
溫度                       37℃
空氣條件      5% CO2，,95% AIR
生長代數(shù)    P4
凍存條件    培養(yǎng)基70%、血清20%、DMSO10%
四、組成：
組份    規(guī)格
細(xì)胞一瓶    T25
細(xì)胞培養(yǎng)與操作說明    1份
五、細(xì)胞接收后的操作流程與注意事項
1.如果細(xì)胞為貼壁細(xì)胞，，而收到時呈懸浮或者部分懸浮的狀態(tài)，請將懸浮的細(xì)胞即時離心，加15%血清的*培養(yǎng)基到新的培養(yǎng)皿/瓶繼續(xù)培養(yǎng)3天；同時原培養(yǎng)瓶中剩下的貼壁細(xì)胞更換為15%血清的*培養(yǎng)基，培養(yǎng)3天。3天后若原瓶或者新瓶中的細(xì)胞都沒有出現(xiàn)增殖而是繼續(xù)脫落死亡，請及時實驗室，技術(shù)人員會跟進(jìn)解決
2.貼壁細(xì)胞生長緩慢：適當(dāng)提高血清濃度（zui高不能超過20%），或可根據(jù)該細(xì)胞生長密度，考慮胰酶消化后，轉(zhuǎn)移到新的培養(yǎng)瓶繼續(xù)培養(yǎng)
3.生長不均：貼壁細(xì)胞若出現(xiàn)分布不均，成島狀生長，可將細(xì)胞進(jìn)行消化，重懸打散細(xì)胞，加入新鮮培養(yǎng)基進(jìn)行培養(yǎng)

六、細(xì)胞常規(guī)培養(yǎng)傳代流程（請嚴(yán)格遵照無菌操作）
1．吸出原培養(yǎng)瓶中的培養(yǎng)基，PBS緩沖液潤洗細(xì)胞兩次，加2~3 ml  0.25%胰酶進(jìn)行消化細(xì)胞（注意把握消化時間，通常控制在1-2min）
2．鏡下觀察消化情況，在細(xì)胞邊緣縮小，貼壁松動時（不建議消化到細(xì)胞漂浮）去掉胰酶，加6~8ml *培養(yǎng)基，輕輕吹打細(xì)胞層，盡量把細(xì)胞層吹落，吹散
3．取部分細(xì)胞懸液轉(zhuǎn)移到新的培養(yǎng)皿/瓶中，添加適當(dāng)?shù)?培養(yǎng)基，，把細(xì)胞懸液打勻，于培養(yǎng)箱中培養(yǎng)
4．注意培養(yǎng)基PH值變化情況，定期換液（每周2-3次），待細(xì)胞密度達(dá)到80%以后重復(fù)1項操作或者凍存
特別注意：（如使用公共實驗室或初次接觸細(xì)胞培養(yǎng)，建議添加雙抗培養(yǎng)）
1.收到細(xì)胞后請盡快更換為含15%血清的新鮮培養(yǎng)基，如因特殊情況需要繼續(xù)使用原瓶培養(yǎng)基，請在原瓶培養(yǎng)基中額外添加10%的血清（原瓶培養(yǎng)基的繼續(xù)使用時間zui長不宜超過72小時）
2.貼壁細(xì)胞收到當(dāng)天切忌立刻消化，請將細(xì)胞放置培養(yǎng)箱孵育過夜到第二天再做消化傳代
如簽收時出現(xiàn)培養(yǎng)瓶壁破裂，漏液等情況請及時做好照片記錄并實驗室
TNS-sf (Trypsin Neutralization Solution-serum free)
Enzyme-Free Cell Dissociation Solution
Cell Freezing Medium
Serum-Free Cell Freezing Medium
Human Pluripotent Stem Cell Cryopreservation Medium
Human Pluripotent Stem Cell Cryopreservation Medium,
Trypan Blue, 0.4%
Dulbecco’s Phosphate-Buffered Saline
Hank's Balanced Salt Solution
Poly-L-Lysine 1 mg/ml
Poly-L-Lysine 10 mg/ml
0.2% Gelatin Solution
Fetal Bovine Serum, Human Cell Tested
Penicillin/Streptomycin Solution
Penicillin/Streptomycin Solution
Antimycotic Solution
Antibiotic/Antimycotic Solution
Cell Culture Grade Water
Myocyte Detachment Solution
Bovine Pituitary Extract
Bovine Pituitary Extract
Insulin-Transferrin-Selenium, 100x
L-Glutamine Solution, 200 mM
100X Non-Essential Amino Acids
Human Germ Layer Detection Kit
Oil Red O Staining Kit
StemDS? Human Embryonic Stem Cell Dissociation Solution
Bovine Plasma Fibronectin
Human Cellular Fibronectin
 Bovine Plasma Vitronectin
"Recombinant Human Serum Albumin
"

Bovine Plasma Fibronectin
Collage I-Cell Culture Surface Coating Kit
Poly-L-Lysine 1 mg/ml
Poly-L-Lysine 10 mg/ml

Human Pluripotent Stem Cell Growth Medium
Xeno-free Human Pluripotent Stem Cell Growth Medium
Animal Component-free Human Pluripotent Stem Cell Growth Medium
StemDS? Human Embryonic Stem Cell Dissociation Solution
Human Pluripotent Stem Cell Cryopreservation Medium
Human Pluripotent Stem Cell Cryopreservation Medium,
Mouse Embryonic Fibroblasts
Mouse Embryonic Fibroblasts-mitomicine treated
Mouse Embryonic Fibroblasts- Mitomycin C treated from CD1 mouse 
Mouse Embryonic Fibroblasts from CF1 mouse 
Mouse Embryonic Fibroblasts-Mitomycin C treated from CF1 mouse 
Human Dermal Fibroblasts-Fetal-Mitomycin C treated
Mouse Embryonic Fibroblasts-Conditioned Medium
bFGF-Stimulated Mouse Embryonic Fibroblasts Conditioned Medium
Ultra-Low Binding Culture Plate
Ultra-Low Binding Culture Plate (6-well) 
Ultra-Low Binding Culture Plate (24-well) 
Ultra-Low Binding Culture Plate (24-well) 
Recombinant Human Fibroblast Growth Factor-basic
"Recombinant Human Epidermal Growth Factor
"
"Recombinant Human Bone Morphogenetic Protein-2
"
"Recombinant Human Bone Morphogenetic Protein-4
"
"Recombinant Human Bone Morphogenetic Protein-7
"
"Recombinant Human NOGGIN
"

Human Amniotic Mesenchymal Stromal Cells 
Human Chorionic Mesenchymal Stromal Cells 
Human Mesenchymal Stem Cells-bone marrow