A549細胞株

A549細胞株 人非小細胞肺癌細胞系是1972年由GiardDJ通過肺癌組織移植培養建系的，源自一位58歲的白人男性。A549能通過胞苷二磷脂酰途徑合成富含不飽和脂肪酸的；角蛋白陽性。

細胞名稱：人非小細胞肺癌細胞

細胞別稱： A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549-ATCC; hA549

細胞簡稱：A549細胞株

產品貨號：TCH-C116

種屬來源：人

組織來源：肺

特征：肺癌

細胞形態：上皮細胞樣

生長特性：貼壁生長

培養體系：D/F12+10%FBS+1%P/S

A549細胞培養基貨號：TCH-G116

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存

質量檢測：細菌、真菌、支原體檢測均為陰性

實驗室儀器及培養環境：CO2培養箱 細胞培養瓶 細胞培養皿

參考文獻："Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed:

Giard DJ, et al. In vitro c*tion of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383

St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830

Horikami SM, et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475"