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綠膿菌素測定用培養(yǎng)基（PDP瓊脂培養(yǎng)基）

King Medium A

250g

用于綠膿桿菌的綠膿菌素測定試驗（GB標準）

用途：用于綠膿桿菌的綠膿菌素測定試驗

綠膿菌素測定用培養(yǎng)基（PDP瓊脂培養(yǎng)基） 配方：（g/L）

蛋白胨
氯化鎂
硫酸鉀
瓊脂
pH值7.4 ± 0.1

20.0
1.4
10.0
18.0
25℃

原理：

蛋白胨和空白提供碳氮源；氯化鎂和硫酸鉀促進綠膿色素和熒光素的產(chǎn)生；瓊脂是培養(yǎng)基的凝固劑。

用法：

稱取本品 49.4g,另稱取 10g 空白,溶解于1000ml蒸餾水中,分裝,121℃高壓滅菌 15 分鐘,備用。

質(zhì)量控制：

在37培養(yǎng)22-24小時。

質(zhì)控菌株	ATCC	生長情況	色素情況
金葡萄球菌	25923	-
大腸桿菌	25922	-/+	無色
綠膿假單胞菌	14028	+++	綠色

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